Manuscript preparation

The information below detail the section headings that you should include in your manuscript and what information should be within each section.

Title page
The title page should include the following information.
  1)   Manuscript title, which should be concise but informative.
  2)   Each author’s name (given name, middle name, and surname) followed by the highest academic degree.
  3)   The name of the department(s) and institution(s) where the work was conducted.
  4)   A running title of fewer than 60 characters.
  5)   Indication of the corresponding author and complete mailing address, telephone and e-mail address of the corresponding

The abstract should concisely present the hypothesis being tested, general methods, results, and conclusions. The Abstract should not exceed 300 words. Please minimize the use of abbreviations and do not cite references in the abstract.

Four to eight keywords representing the main content of the article.

This section should contain a clear statement of the aims of the work or of the hypotheses being tested. A brief account of the relevant background that supports the rationale of the study should also be given.

The Methods should provide enough details for reproduction of the findings. Protocols for new methods should be included, but well-established methodological procedures may simply be referenced. A full description of the methods should be included in the manuscript itself rather than in a supplemental file.
Ethics approval
Original studies in animals have been carried out in accordance with the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the U.S. National Institutes of Health, and were approved by the Institution’s Animal Care and use Committee or local equivalent. authors should also report the source, species, strain, sex, age, randomization, blinding, and husbandry of the animals.
For studies involving human subjects, authors must affirm that they have been carried out in accordance with the Declaration of Helsinki and approved by the Institutional Review Board(s) or equivalent ethics committee.

In research involving statistical analysis, please include the following: (a) the hypotheses in clearly stated form, (b) the statistical tests used, cited by name, for each data set, (c) the use of either one-tailed or two-tailed tests, and (d) the test value, degree(s) of freedom, and probability for every significant and important nonsignificant result. Use standard deviations rather than standard errors of the mean. For novel or not well-known tests, provide a citation.

Authors should present and interpret the results and conclusions in an appropriate and comprehensive manner, clearly explaining the results and outcomes of their study. Incomplete interpretation of results may result in rejection of the manuscript.

This should explore the significance of the results of the work, and present reproducible procedure. Extensive citations and discussion of published literature should be avoided.This section can be combined with the ‘Result’ section if necessary.

This should state clearly the main conclusions and provide an explanation of the importance and relevance of the case, data, opinion, database or software reported.

List of abbreviations
If abbreviations are used in the text they should be defined in the text at first use, and a list of abbreviations should be provided.

All manuscripts must contain the following sections under the heading 'Declarations':
Competing interests
All financial and non-financial competing interests must be declared in this section.
Authors' contributions
The individual contributions of authors to the manuscript should be specified in this section. Guidance and criteria for authorship can be found in our editorial policies.
Include all funding sources, grants, and other financial support received for the work represented in the manuscript (both internal and external). Include the granting institution, and grant number (if relevant). Individual who has substantially contributed to the study for important intellectual content, or who was involved in the article’s drafting the manuscript or revising must also be acknowledged.

Consent for publication
If your manuscript contains any individual person’s data in any form (including individual details, images or videos), consent to publish must be obtained from that person, or in the case of children, their parent or legal guardian. All presentations of case reports must have consent to publish.
If your manuscript does not contain data from any individual person, please state “Not applicable” in this section.

References should be cited with Arabic numerals in brackets and numbered in the order cited and presented in a separate section at the end of the document. Every reference should be assigned its own unique number. References should not be repeated in the list, with each mention given a different reference number, nor should multiple references be combined under a single reference number.
All published or accepted manuscript can be cited. Unavailable or unpublished work and Personal communications are not allowed to be cited in the reference list
References in the text should be formatted as samples indicated below.
Chatel JM, Bernard H, Orson FM. Isolation and characterization of two complete Ara h 2 isoforms cDNA. Int Arch Allergy Immunol 2003; 131:14–18.
Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM, et al. Orthogonal cas9 proteins for RNA-guided gene regulation and editing. Nat Methods 2017; 10(11), 1116.
Duke JA. Handbook of phytochemical constituents of GRAS herbs and other economic plants. Boca Raton (FL): CRC Press 2001.
Grigg W, Moran R, Kuang M. National Indian education study. Washington (DC): National Center for Education Statistics; 2010. NCES publication 2010-462.
Online document
Doe J. Title of subordinate document. In: The dictionary of substances and their effects. Royal Society of Chemistry. 1999. of subordinate document. Accessed 15 Jan 1999.

Figure legends
Include a brief separate Legend for each figure at the end of the text. The title of the figure should appear in the Legend, not on the figure itself. Use the Legend to identify all abbreviations, lines, bars, etc. used in the figure.

Tables and figures
All tables, figures should be numbered consecutively as they appear in the text. All figures and illustrations must include legends
Figures should be submitted as separated files in high resolution (at least 600dpi). The figures should be sized to column width (8.5 cm or 17.5 cm) and can be provides in TIFF, EPS or JPGE, PPT format.
Please submit tables as editable text and not as images. Tables can be placed either next to the relevant text in the article, or on separate page at the end. Be sparing in the use of tables and ensure that the data presented in them do not duplicate results described elsewhere in the article. Please avoid using vertical rules and shading in table cells.